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umbilical cord matrix derived mscs (PromoCell)

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PromoCell umbilical cord matrix derived mscs
Umbilical Cord Matrix Derived Mscs, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/umbilical+cord+matrix+derived+mscs/Human+Mesenchymal+Stem+Cells+from+Umbilical+Cord+Matrix/pm40818327-47-0-5
Average 94 stars, based on 57 article reviews

umbilical cord matrix derived mscs - by Bioz Stars, 2026-09

94/100 stars

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Cell Culture:

Article Title: Directed differentiation of human mesenchymal stromal cells into functional endothelial cells having IKAROS-mediated immune tolerance properties.
Article Snippet: We report an efficient method for the directed differentiation of mesenchymal stromal cells derived from embryonic stem cells (EMSCs) into functional endothelial cells (EC), addressing the challenges of direct conversion, such as cellular senescence.. We found that transduction of one transcription factor, ER71, was required for the efficient derivation of induced endothelial cells from EMSCs (MiECs).. Addition of transforming growth factor-β inhibitor (SB431542), vascular endothelial growth factor, and ascorbic acid to the culture media enhanced the differentiation efficiency.

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MSC.sTRAIL induces higher apoptosis levels in colorectal cancer cells than MSC.FL-TRAIL. ( a ) Murine MSCs (mMSCs) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA. ( b ) Human MSCs (hMSCs) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA. ( c ) Different types of MSCs (adipose tissue-derived - AT, bone marrow-derived - BM, and umbilical cord-derived - UC) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were passed through a 0.45 μm filter before TRAIL levels were measured by ELISA. ( d ) Apoptosis was measured in different types of MSCs, adipose tissue-derived (AT), bone marrow-derived (BM) and umbilical cord-derived (UC) and mouse (mMSC) MSCs that were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). ( e ) Survival was measured in HT-29 cells after exposure to supernatants from MSC.FL-TRAIL (red) and MSC.sTRAIL (yellow) at increasing concentrations of sTRAIL. For MSC.FL-TRAIL supernatants equal volumes of supernatant were used for each corresponding sTRAIL concentration. ( f ) Apoptosis was measured in HT-29 cells after exposure to supernatants from MSC.FL-TRAIL (red) and MSC.sTRAIL (yellow) at increasing concentrations of sTRAIL. For MSC.FL-TRAIL supernatants equal volumes of supernatant were used for each corresponding sTRAIL concentration. ( g ) Survival was measured in HT-29 cells that were co-cultured with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) in 24-well plates fitted with transwell tissue culture inserts. ( h ) Apoptosis was measured at 24 h and 72 h in HT-29 cells. For this HT-29 cells were co-cultured with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) in 24-well plates fitted with transwell tissue culture inserts. ( i ) Morphologically altered cells were measured by flow cytometry (changes in FSC/SSC) after mixing HT-29 cells with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at a ratio of 10:1. ( j ) Apoptosis measurements of HT-29 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at a ratio of 10:1. ( k ) Apoptosis measurements of Colo205 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at different ratios (5:1; 10:1; 100:1). Representative images of Colo205 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) are shown on the right side of the panel. MSCs are pointed out by blue arrows, apoptotic cells by red arrows. Scale bar is 30 μm. Values represent mean ± SE.

Journal: Cancers

Article Title: MSC.sTRAIL Has Better Efficacy than MSC.FL-TRAIL and in Combination with AKTi Blocks Pro-Metastatic Cytokine Production in Prostate Cancer Cells

doi: 10.3390/cancers11040568

Figure Lengend Snippet: MSC.sTRAIL induces higher apoptosis levels in colorectal cancer cells than MSC.FL-TRAIL. ( a ) Murine MSCs (mMSCs) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA. ( b ) Human MSCs (hMSCs) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were either taken as crude supernatants (c), centrifuged (s) or filtered through a 0.45 μm filter (f) before TRAIL levels were measured by ELISA. ( c ) Different types of MSCs (adipose tissue-derived - AT, bone marrow-derived - BM, and umbilical cord-derived - UC) were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). The resulting medium supernatants were passed through a 0.45 μm filter before TRAIL levels were measured by ELISA. ( d ) Apoptosis was measured in different types of MSCs, adipose tissue-derived (AT), bone marrow-derived (BM) and umbilical cord-derived (UC) and mouse (mMSC) MSCs that were transduced with a control adenoviral vector (ctrl), a vector expressing FL-TRAIL (FL) or a vector expressing sTRAIL (sT). ( e ) Survival was measured in HT-29 cells after exposure to supernatants from MSC.FL-TRAIL (red) and MSC.sTRAIL (yellow) at increasing concentrations of sTRAIL. For MSC.FL-TRAIL supernatants equal volumes of supernatant were used for each corresponding sTRAIL concentration. ( f ) Apoptosis was measured in HT-29 cells after exposure to supernatants from MSC.FL-TRAIL (red) and MSC.sTRAIL (yellow) at increasing concentrations of sTRAIL. For MSC.FL-TRAIL supernatants equal volumes of supernatant were used for each corresponding sTRAIL concentration. ( g ) Survival was measured in HT-29 cells that were co-cultured with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) in 24-well plates fitted with transwell tissue culture inserts. ( h ) Apoptosis was measured at 24 h and 72 h in HT-29 cells. For this HT-29 cells were co-cultured with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) in 24-well plates fitted with transwell tissue culture inserts. ( i ) Morphologically altered cells were measured by flow cytometry (changes in FSC/SSC) after mixing HT-29 cells with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at a ratio of 10:1. ( j ) Apoptosis measurements of HT-29 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at a ratio of 10:1. ( k ) Apoptosis measurements of Colo205 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) at different ratios (5:1; 10:1; 100:1). Representative images of Colo205 cells mixed with control MSCs (ctrl), MSC.FL-TRAIL (FL) or MSC.sTRAIL (sT) are shown on the right side of the panel. MSCs are pointed out by blue arrows, apoptotic cells by red arrows. Scale bar is 30 μm. Values represent mean ± SE.

Article Snippet: Human bone marrow derived MSCs were from Lonza, human adipose derived stem cells were from Amsbio (Cambridge, MA, USA) and human umbilical cord derived MSCs were from Promocell (Heidelberg, Germany).

Techniques: Transduction, Control, Plasmid Preparation, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Concentration Assay, Cell Culture, Flow Cytometry